Updated in 1/17/2011 4:18:15 PM      Viewed: 149 times      (Journal Article)
Microscopy research and technique 63 (1): 18-22 (2004)

Practical limits of resolution in confocal and non-linear microscopy

G Cox , CJ Sheppard
ABSTRACT
AbstractCalculated and measured resolution figures are presented for confocal microscopes with different pinhole sizes and for nonlinear (2-photon and second harmonic) microscopes. A modest degree of super-resolution is predicted for a confocal microscope but in practice this is not achievable and confocal fluorescence gives little resolution improvement over widefield. However, practical non-linear microscopes do approach their theoretical resolution and therefore show no resolution disadvantage relative to confocal microscopes in spite of the longer excitation wavelength.Copyright 2003 Wiley-Liss, Inc.
ISSN: 1059-910X