Updated in 7/28/2011 3:39:50 PM      Viewed: 535 times      (Proceedings)
Tissue Engineering and Regenerative Medicine International Society Asia Pacific Meeting 2011

BMP-2 Embedded Atelocollagen Scaffold For Tissue Engineered Cartilage Cultured In The Medium Containging Insulin And Triiodothyronine – A New Protocol For 3D In Vitro Culture Of Human Chondrocytes

EC Ko , Y Fujihara , T Ogasawara , Y Asawa , S Nishizawa , S Nagata , T Takato , K Hoshi
ABSTRACT
Background
When the chondrocytes are isolated from the native cartilage and proliferate in vitro, they soon lose their original ability to express GAG and type II collagen, which is termed dedifferentiation, or decrease cell viability.
Methods
We first examined the in vitro cartilage regeneration of tissue-engineered pellets that consisted of human auricular chondrocytes and atelocollagen and that were incubated in vitro under stimulation with BMP-2, insulin and T3. We then examined the administration of those growth factors into the scaffold or in the medium and explored the possibility that the atelocollagen, the hydrogel scaffold of the chondrocytes, may function for drug delivery of the factors.
Results
BMP-2 in the atelocollagen with the supplement of insulin and T3 in the medium could not only produce a greater GAG matrix in a shorter period but also sustain cell viability with lower mortality. The insulin in the medium could be better administered only for 2 weeks, rather than 3 weeks, which would save time and cost, hence shortening the in vitro culture of chondrocytes.
Conclusions
Our protocol of mixing BMP-2 into the atelocollagen with the supplement of insulin and T3 hormone might provide a new insight into the development of tissue engineering in chondrogenesis.
Keywords: chondrocytes, tissue engineering, cartilage, insulin, atelocollagen
Notes
Date: 3th August 2011 Time:1745-1800
Venue: Waterfront Ballroom II, Grand Copthorne Waterfront Hotel

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