Updated in 7/25/2011 11:20:06 AM      Viewed: 456 times      (Proceedings)
Tissue Engineering and Regenerative Medicine International Society Asia Pacific Meeting 2011

Haematopoietic Stem Cell Marking By An Intronic Runx1 Enhancer

M Osato
There has been considerable interest in identifying a cis-regulatory element that targets gene expression to stem cells. Such an element, so called “stem cell enhancer”, holds the promise of providing important insights into the transcriptional programs responsible for “stemness”, and also serves as a molecular handle for stem cell-specific marking, transgenesis and gene targeting, and consequently become an invaluable powerful tool for research on stem cell biology. A series of candidate enhancers have been identified for haematopoietic stem cells (HSC). However, none of them have met the stringent requirements as a HSC enhancer. We have recently succeeded in identifying an intronic enhancer in the Runx1 gene which is critical for the generation and maintenance of HSC. The element, named Runx1 +24mCNE, was discovered through a combinatorial in silico approach and subsequent in vivo verification using zebrafish and mouse transgenic models. Runx1 +24mCNE is specifically active in HSC and hence the most ideal bona fide HSC enhancer. The Runx1 HSC enhancer also marks haemogenic endothelial cells (EC), the immediate precursor of HSC in embryos. The precise detection of haemogenic EC would help to better understand the developmental machinery of HSC generation and facilitate the development of method to produce HSC from embryonic stem (ES) cells. The Runx1 HSC enhancer is likely to be active in leukaemia stem cells (LSC). Because of their rarity, LSC are difficult to detect using currently available methods. LSC marking by the Runx1 HSC enhancer would serve as a powerful tool for cancer research and drug development.
*Keynote Presentation
Date: 5th August 2011 Time:1545-1615
Venue: Riverfront Ballroom, Grand Copthorne Waterfront Hotel

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